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facs buffer  (Thermo Fisher)


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    Thermo Fisher facs buffer
    Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer/pm42129323-254-13-41?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    facs buffer - by Bioz Stars, 2026-07
    99/100 stars

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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Journal: bioRxiv

    Article Title: A Single-Cell Atlas of the Mouse Dural Meninges Reveals Pervasive Sex Differences Across Cellular Compartments

    doi: 10.64898/2026.05.12.721894

    Figure Lengend Snippet: A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Article Snippet: The cell pellets were resuspended in ice-cold FACS buffer (0.1M PBS; 1mM EDTA (Fisher Scientific) and 1% BSA (Equitech-Bio; BAH70); pH 7.4).

    Techniques: Isolation, Expressing, Quantitative Proteomics